Table 1.

Primer sequences used for scRT-PCR experiments^a

	Gene	Pairs of primers (from 5′ to 3′)
		GenBank no.	First-round PCR	Second-round PCR
GAPDH	AF106860.2	Sense	CCTGCACCACCAACTGCTTAGC	CCTGCACCACCAACTGCTTAGC
		Antisense	CTCGGCCGCCTGCTTCAC	ATGTCAGATCCACAACGGATACATTGG
RXFP3	NM_001008310.1	Sense	CAAGCTCCTGGGTTGGGACC	CAAGCTCCTGGGTTGGGACC
		Antisense	GCATTAAGTGGCGCCAGGGC	GGCGCACTAAGCAGTAGAGGAT
KCNQ2	NM_133322.1	Sense	GTGCTGATTGCCTCCATTGCTG	GTGCTGATTGCCTCCATTGCTG
		Antisense	CCAATGGTTGTCAGGGTGATCAG	GGCCAGGATGAGGCAGAGG
KCNQ3	NM_031597.4	Sense	CTATTCGGACCACATCTCACCC	CTATTCGGACCACATCTCACCC
		Antisense	ACTACAGTACCAGGAGTCAGCC	CATAGGCATGGATCCACTGGG
KCNQ4	XM_233477.8	Sense	GACGATTACACTGACGACCATTGG	GACGATTACACTGACGACCATTGG
		Antisense	CCAGGGCAGAAGGAGGCAC	CTCAAACAAGAGGGCCAGCTC
KCNQ5	XM_001071249.3	Sense	GCTGGGCTCCGTGGTTTACG	GCTGGGCTCCGTGGTTTACG
		Antisense	CTTCTGCACTTTGGTGGGGCTG	GCTTGCTGCCTCCCCTTGTTC

^aGenBank accession number and sequences of primers used for each of the examined genes in both rounds of PCR.