Table 2.
Synaptic input to PVN MNCs is unaffected by RLN3 and a selective RXFP3 agonista
| Inhibitory |
Excitatory |
|||||||
|---|---|---|---|---|---|---|---|---|
| Male (N = 11) |
Female (N = 9) |
Male (N = 11) |
Female (N = 9) |
|||||
| Baseline | RLN3 | Baseline | RLN3 | Baseline | RLN3 | Baseline | RLN3 | |
| RLN3 | ||||||||
| Spontaneous synaptic activity | ||||||||
| Frequency (Hz) | 8.9 ± 5.4 | 9.2 ± 5.7 | 7.8 ± 5.9 | 7.9 ± 5.7 | 1.3 ± 1.0 | 1.2 ± 0.6 | 0.8 ± 0.3 | 0.9 ± 0.4 |
| Mean amplitude (pA) | 33.6 ± 9.6 | 32.7 ± 9.9 | 31.0 ± 5.4 | 30.2 ± 4.6 | 18.9 ± 2.7 | 18.9 ± 3.4 | 21.6 ± 3.5 | 21.3 ± 3.5 |
| Mean rise time (ms) | 2.8 ± 0.4 | 2.8 ± 0.3 | 2.7 ± 0.3 | 2.7 ± 0.2 | 2.4 ± 0.2 | 2.3 ± 0.1 | 2.4 ± 0.2 | 2.4 ± 0.2 |
| Decay time constant (ms) | 5.5 ± 1.3 | 5.6 ± 1.3 | 5.0 ± 1.2 | 4.9 ± 1.0 | 2.3 ± 0.8 | 2.3 ± 0.6 | 2.1 ± 0.5 | 2.0 ± 0.5 |
| Male (N = 9) |
Female (N = 10) |
Male (N = 9) |
Female (N = 10) |
|||||
| Baseline | RLN3 | Baseline | RLN3 | Baseline | RLN3 | Baseline | RLN3 | |
| Miniature synaptic activity | ||||||||
| Frequency (Hz) | 10.1 ± 6.0 | 9.5 ± 5.3 | 6.4 ± 4.4 | 6.4 ± 4.5 | 1.5 ± 1.6 | 1.1 ± 0.6 | 0.9 ± 0.4 | 0.9 ± 0.4 |
| Mean amplitude (pA) | 25.8 ± 3.8 | 26.1 ± 4.0 | 26.1 ± 5.6 | 25.7 ± 5.3 | 19.6 ± 5.3 | 18.9 ± 3.6 | 16.3 ± 3.4 | 16.3 ± 3.2 |
| Mean rise time (ms) | 2.6 ± 0.2 | 2.6 ± 0.2 | 2.6 ± 0.2 | 2.6 ± 0.2 | 2.3 ± 0.1 | 2.3 ± 0.1 | 2.3 ± 0.2 | 2.4 ± 0.1 |
| Decay time constant (ms) | 4.7 ± 0.8 | 4.7 + 0.7 | 4.9 ± 0.9 | 5.1 ± 0.8 | 2.1 ± 0.9 | 1.8 ± 0.7 | 2.0 ± 0.6 | 2.0 ± 0.5 |
| Male (N = 10) |
Female (N = 9) |
Male (N = 10) |
Female (N = 9) |
|||||
| Baseline | A2 | Baseline | A2 | Baseline | A2 | Baseline | A2 | |
| RXFP3-A2 | ||||||||
| Spontaneous synaptic activity | ||||||||
| Frequency (Hz) | 8.6 ± 7.3 | 8.4 ± 7.1 | 11.3 ± 6.7 | 11.0 ± 6.5 | 1.0 ± 0.8 | 1.0 ± 0.6 | 1.1 ± 0.6 | 1.1 ± 0.5 |
| Mean amplitude (pA) | 32.0 ± 5.5 | 31.8 ± 6.3 | 34.1 ± 7.4 | 33.0 ± 7.2 | 19.5 ± 4.8 | 20.9 ± 6.3 | 19.7 ± 2.3 | 20.0 ± 3.0 |
| Mean rise time (ms) | 2.8 ± 0.3 | 2.7 ± 0.2 | 2.8 ± 0.2 | 2.8 ± 0.2 | 2.4 ± 0.2 | 2.4 ± 0.2 | 2.3 ± 0.1 | 2.3 ± 0.1 |
| Decay time constant (ms) | 5.9 ± 1.1 | 5.7 ± 1.1 | 5.8 ± 1.3 | 5.9 ± 1.2 | 3.0 ± 1.6 | 2.7 ± 1.2 | 2.1 ± 0.5 | 2.1 ± 0.4 |
| Male (N = 10) |
Female (N = 8) |
Male (N = 10) |
Female (N = 8) |
|||||
| Baseline | A2 | Baseline | A2 | Baseline | A2 | Baseline | A2 | |
| Miniature synaptic activity | ||||||||
| Frequency (Hz) | 6.8 ± 3.2 | 6.7 ± 3.0 | 7.8 ± 3.7 | 7.3 ± 3.7 | 1.0 ± 0.5 | 1.1 ± 0.5 | 0.7 ± 0.4 | 0.7 ± 0.4 |
| Mean amplitude (pA) | 28.7 ± 5.7 | 29.2 ± 5.6 | 32.5 ± 3.2 | 32.8 ± 3.5 | 19.6 ± 4.1 | 19.8 ± 3.2 | 19.4 ± 1.1 | 19.3 ± 2.5 |
| Mean rise time (ms) | 3.1 ± 0.4 | 3.0 ± 0.3 | 3.1 ± 0.8 | 3.0 ± 0.3 | 2.8 ± 0.3 | 2.9 ± 0.2 | 2.8 ± 0.2 | 2.8 ± 0.2 |
| Decay time constant (ms) | 4.7 ± 1.1 | 4.7 ± 0.9 | 4.0 ± 0.5 | 4.2 ± 0.4 | 2.2 ± 1.1 | 2.1 ± 1.0 | 2.0 ± 0.7 | 1.9 ± 0.4 |
aSpontaneous and miniature postsynaptic currents recorded from male and female MNCs under two experimental conditions: baseline and after application of RLN3 or RXFP3-A2. No significant effect of treatment, sex, or interaction of treatment and sex were observed in all of the cases analyzed (repeated-measures two-way ANOVA, p > 0.05).