Figure 6.

Lrp4 is mainly expressed in astrocytes, not neurons, oligodendrocytes, or microglia. A, Knock-in of the 2A-CreERT2 cassette in exon 38 of the Lrp4 allele. B, The protocol of Tam administration. Lrp4-CreERT2;Ai9 mice at P42 were treated with Tam (100 mg/kg, i.p., three times, once every other day) and killed at P56. Shown are brain sections of the cortex (C,D), hippocampus (E,F), and enlarged views of cortical cells costained with the different maker (G), except that the Slc1a2⁺ cells are labeled by Slc1a2-GFP (in Slc1a2-GFP; Lrp4-CreERT2;Ai9 mice). tdTomato⁺ cell costained with astrocytes marker Aldoc and S100β (arrow), but not the with NeuN, Oligo2, and Iba1 (arrowhead). Scale bar, 20 µm. H, Quantification of double-positive cells. n = 3 for each group. Td, tdTomato. Data are mean ± SEM.