Figure 8.

Sema3A-dependent dendritic arborization relies on the FARP2 GEF and downstream Rac1 GTPase activation. A, Representative confocal micrographs of dissociated primary neurons obtained from Farp2 KO animals. The neurons were treated with 5 nm AP, treated with 5 nm Sema3A, transfected with Farp2-HA cDNA and treated with 5 nm AP, transfected with Farp2-HA cDNA and treated with 5 nm Sema3A and transfected with Farp2-HA cDNA, treated with 5 nm Sema3A + 2.5 uM EHT for 24 h. B, Sholl analysis of dendritic intersections in the five different groups. Data are mean ± SEM from three independent cultures. **, ^#p < 0.01 (two-way ANOVA with post hoc Tukey test). **Statistical difference between Farp2 KO+Farp2-HA and Farp2 KO+Farp2- HA+Sema3A. ^#Statistical difference between Farp2 KO+Farp2-HA+Sema3A and Farp2 KO+Farp2-HA+Sema3A + EHT. Scale bars: A, 25 µm.