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(A) Transformants of cdc33-1 mutant F696 harboring empty vector YEplac195, hcCDC33 plasmid (p3351), or hc plasmids with the indicated WT (p4504) or mutant (pSG48 or pSG49) DED1 alleles were examined for rates of colony formation at the indicated temperatures as in Figure 3B. (B) Western blot analysis of the strains in (A) conducted as in Figure 3D using the indicated antibodies.
