Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus. musculus) | C57BL/6(J) | Jackson lab | Stock No: 000664 | |
| Genetic reagent (Mus. musculus) | C57BL/6(N) | Jackson lab | Stock No: 005304 | |
| Genetic reagent (Mus. musculus) | Ccr2-/- | Gift of Marco Prinz, (University of Freiburg) | ||
| Genetic reagent (Mus. musculus) | Rag2–/–γc–/– | Gift of Tilman Brummer (University of Freiburg) | BALB/c background | |
| Genetic reagent (Mus. musculus) | Cx3cr1gfp/+ | Gift of Steffen Jung (Weizmann Institute of Science) |
||
| Genetic reagent (Mus. musculus) | Myd88-/- | Deshmukh et al., 2012 | ||
| Genetic reagent (Mus. musculus) | sDMDMm2 | Clean Mouse Facility, University of Bern, Switzerland | C57BL/6(J) background | |
| Antibody | anti-mouse CD45 eFluor450 | Invitrogen | Cat: 48-0451-82 Clone: 30-F11 |
1:300 |
| Antibody | anti-mouse CD11b PE-Cy7 | Invitrogen | Cat: 25-0112-82 Clone: M1/70 |
1:3000 |
| Antibody | anti-mouse Ly6G FITC | BD Pharmingen | Cat: 551460 Clone: 1A8 |
1:200 |
| Antibody | anti-mouse Ly6C PerCP-Cy5.5 | BD Pharmingen | Cat: 560525 Clone: AL-21 |
1:500 |
| Antibody | anti-mouse CD64 PerCP/Cy5.5 | Biolegend | Cat: 139301 Clone: X54-5/7.1 |
1:100 |
| Antibody | anti-mouse CD11c APC | BD Pharmingen | Cat:561119 Clone HL3 |
1:100 |
| Antibody | mouse MHC class II eFluor450 | eBioscience | Cat: 48-5320-82 Clone: AF6120.1 |
1:400 |
| Antibody | anti-mouseCD3e | Invitrogen | Cat: 12-0031-82 Clone: 145–2 C11 |
1:200 |
| Antibody | anti-mouse Ly6C PE | Invitrogen | Cat: 12-5932-80 Clone: HK1.4 |
1:1000 |
| Antibody | Anti-mouse pSTAT1 AF647 | BD Biosciences | Cat:612597 Clone: 4a |
|
| Chemicalcompound/drug | liposomal clodronate | Clodrosome Encapsula NanoSciences |
Cat: CLD-8909 | |
| Chemicalcompound/drug | PKH26 Red | Sigma Aldrich | Cat: PKH26GL | |
| Software | GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | Version 8 | |
| Software | Kaluza analysis | https://www.beckman.de/flow-cytometry/software/kaluza |