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. 2020 Jun 17;12(12):12324–12341. doi: 10.18632/aging.103441

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Copyright © 2020 Battaglia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Comparison of DE miRNA sets obtained by two normalization methods for RT-qPCR. Differential expression analysis of miRNAs from FF EVs, comparing older women to younger women and using two normalization methods revealed 64 DE miRNAs (gFC), without considering the number of EVs, and 80 DE miRNAs (vFC) taking into account the number of vesicles. Comparison between gFC and vFC datasets identified 4 classes of DE miRNAs (A-D) for which we show a possible distribution (represented by vertical bars) within the individual EVs from the FF of the two groups of women according to an EV ratio of 2:1 (older vs younger).