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PLK1 knockdown inhibits TGFβ-mediated differentiation of human myofibroblasts. (A) Schematic depicting establishment of the recombinant plasmid RSV-PLK1-shRNA. (B) A representative image of infected human primary myofibroblast cells. (C–F) The expression of [50] α-SMA was analyzed by WB (C, D), qRT-PCR (E, F), and IF (G). (H) Migration ability of myofibroblasts in the control and PLK1-shRNA-treated groups.
