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. 2020 Jun 7;12(12):11325–11336. doi: 10.18632/aging.103410

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Copyright © 2020 Ogura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Apigenin increases Sirt3 activity in diabetic rats. (A) Western blots of Sirt3, acetylated (ace) (Lys413)-IDH2, IDH2, acetylated (ace) (Lys68)-SOD2, SOD2, and CoxIV from mitochondrial protein extracts obtained from rat renal cortex. (B–D) Densitometric evaluation of Sirt3 to CoxIV (B), Ace (Lys413)-IDH2 to IDH2 (C), and Ace (Lys68)-SOD2 to SOD2 (D) immunoblotting data shown in panel A (n=6). (E) 8-OHdG content in mitochondria (mt) adjusted to mtDNA in the renal cortex (n=6). (F) NAD⁺/NADH ratio in isolated mitochondrial protein extracts from rat renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p<0.01 vs. other groups, n.s; not significant.