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. 2020 Jun 7;12(12):11325–11336. doi: 10.18632/aging.103410

Figure 5.

Figure 5

CD38 knockdown increases Sirt3 activity and intracellular NAD+/NADH ratio in renal proximal tubular cells grown in high glucose. (A) Western blots of CD38, Sirt3, ace (Lys413)-IDH2, IDH2, ace (Lys68)-SOD2, and β-actin in HK-2 cells cultured in low and high glucose after transfection using scrambled or CD38 siRNA. (B–E) Densitometric evaluation of CD38 to β-actin (B), Sirt3 to β-actin (C), Ace-IDH2 to IDH2 (D), and Ace-SOD2 to SOD2 (E) immunoblotting data shown in panel A (n=4). (F) Intracellular NAD+/NADH ratio in cultured HK-2 cells under the above conditions (n= 4). All data represent the mean ± standard deviation (SD). *p<0.01 vs. the indicated group, **p<0.05 vs. the indicated group, n.s; not significant.