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. 2020 Jun 16;12(12):11446–11465. doi: 10.18632/aging.103230

Figure 6.

Figure 6

miR-9 and miR-29a affect neural regeneration after repair of sciatic nerve defect in rats and patients with DM. (A) The positive expression of NGF, BDNF and VEGF in sciatic nerve tissues of normal rats and rats with DM following lentiviral infection detected by immunohistochemistry (× 400) (n = 8). (B) mRNA expression of NGF, BDNF and VEGF in sciatic nerve tissues of normal rats and rats with DM following lentiviral infection detected by RT-qPCR (n = 8). (C, D) The protein expression of NGF, BDNF and VEGF in sciatic nerve tissues of normal rats and rats with DM following lentiviral infection normalized to GAPDH measured by Western blot analysis, with protein band assessed (n = 8). (E) mRNA expression of NGF, BDNF and VEGF in patients with DM detected by RT-qPCR (n = 30). (F, G) The protein expression of NGF, BDNF and VEGF in patients with DM normalized to GAPDH measured by Western blot analysis, with protein band assessed (n = 30); * p < 0.05 vs. the normal group; # p < 0.05 vs. the DM group; @ p < 0.05 vs. the LV-ISL1 + LV-miR-9 group; & p < 0.05 vs. the LV-ISL1 + LV-miR-29a group. Results were measurement data and expressed as mean ± standard deviation; one way analysis of variance was used to analyze data among multiple groups; n = 8; the experiment was repeated three times independently.