Table 2. Primer sequences for RT-qPCR.
| Gene | Sequence (5’– 3’) |
| miR-9 | F: ATAAAGCTAGATAACCGAAAGT |
| R: TTGGGGTGCTCGTGCAGATCGAA | |
| miR-29a | F: ACACTCCAGCTGGGTAGCACCATCTGAAATC |
| R: TGGTGTCGTGGAGTCG | |
| ISL1 | F: TGCCCGCTCCAAGGTGTA |
| R: CCGA AGCGCAAATTCGTC | |
| SHH | F: CTGGCCAGATGTTTT CTGGT |
| R: TAAAGGGGTCAGCTTTTTGG | |
| GLI1 | F: AGTGCTTGCC CAAAGGATGA |
| R: TGACGTGAGACCTGA TCTCGTT | |
| PTCH | F: ATGCTGGAGGAGAACAAGCAA |
| R: CGAGGA CCCCATCATCAGAT | |
| NCF | F: ACC TCT TCG GAC ACT CTG GA |
| R: GTC CGT GGC TGT GGT CTT AT | |
| BDNF | F: GGT CAC AGT CCT GGA GAA AG |
| R: GTC TAT CCT TAT GAA CCG CC | |
| VEGF | F: GGA CAT CTT CCA GGA GTA CC |
| R: CGC ATG ATC TGC ATA GTG AC | |
| U6 | F: ATGACGTCTGCCTTGGAGAAC |
| R: TCAGTGTGCTACGGAGTTCAG | |
| GADPH | F: CTGACATGCCGCCTGGAGA |
| R: ATGTAGGCCATGAGGTCCAC |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR, microRNA; ISL1, Islet factor 1; SHH, Sonic Hedgehog; GLI1, glioma-associated oncogene homolog 1; PTCH, patched tumor suppressor gene; GADPH, glyceraldehyde-phosphate dehydrogenase; NGF, nerve growth factor; BDNF, brain-derived neurotrophic factor; VEGF, vascular endothelial growth factor; F, forward; R, reverse.