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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: AIMS Biophys. 2020 May 26;7(3):144–166. doi: 10.3934/biophy.2020012

Figure 6. Neutrophil and monocyte internalisation of crystalline silica in whole blood by imaging flow cytometry.

Figure 6

A. Percentage of CD14+ monocytes displaying a high DF-BDI signal gated as internalisation high by imaging flow analysis after incubation with crystalline silica in whole blood (n = 5 subjects), * p = 0.006 and ** p = 0.001. B. Example analysis images of CD14+ (green) cells; brightfield (BF), merged DF and internalisation mask (blue) and merged DF/CD14 cell images are shown (from left to right) for examples of internalisation high and low CD14+ cells. C. Percentage of CD16b+ neutrophils displaying a high DF-BDI signal gated as internalisation high by imaging flow analysis after incubation with crystalline silica in whole blood (n = 5 subjects), * p = 0.01. D. Example analysis images of CD16b+ (yellow) cells; BF, merged DF and internalisation mask (blue) and merged DF/CD16 cell example images of internalisation high and low CD16b+ cells are shown. Boxplots display Q1-Q3 with whiskers set at 1.5 x IQR (interquartile range) above the third quartile and 1.5 x IQR below the first quartile, minimum or maximum values that have fallen outside this range are shown as outliers (small black dots). A significant difference from the control was assessed using Tukey’s honest significance test for multiple comparisons following a one way ANOVA.