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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Nat Genet. 2020 Jun 15;52(7):680–691. doi: 10.1038/s41588-020-0637-y

Table 5 |.

Polygenic risk scores and vascular outcomes

Outcome type Outcome T2D PRSdecile n Cases n Controls OR 95%CI lower 95%CI upper P P forlinear trend
Vascular Coronary heart disease 0–10% 2,913 3,924 1.00 Ref Ref - 0.636
10–20% 2,940 3,924 1.01 0.92 1.12 0.811
20–30% 2,958 3,924 0.98 0.89 1.08 0.742
30–40% 2,934 3,924 0.99 0.90 1.09 0.835
40–50% 2,988 3,924 1.01 0.92 1.11 0.801
50–60% 3,001 3,924 0.98 0.90 1.08 0.744
60–70% 2,977 3,924 1.01 0.92 1.10 0.887
70–80% 2,916 3,924 1.02 0.93 1.12 0.632
80–90% 3,032 3,924 0.96 0.88 1.05 0.391
90–100% 3,038 3,924 1.03 0.94 1.12 0.537
Acute ischemic stroke 0–10% 555 6,027 1.00 Ref Ref - 0.070
10–20% 563 6,027 0.90 0.76 1.07 0.238
20–30% 583 6,027 0.98 0.83 1.15 0.782
30–40% 619 6,027 0.98 0.84 1.15 0.821
40–50% 530 6,027 0.99 0.85 1.16 0.924
50–60% 576 6,027 0.99 0.85 1.16 0.941
60–70% 645 6,027 0.97 0.83 1.13 0.672
70–80% 590 6,027 1.04 0.90 1.20 0.611
80–90% 558 6,027 1.05 0.91 1.22 0.494
90–100% 627 6,027 1.02 0.89 1.17 0.784
Peripheral artery 0–10% 1,966 4,871 1.00 Ref Ref - 2.0E-07
disease 10–20% 1,964 4,871 1.00 0.93 1.08 0.927
20–30% 1,948 4,871 1.01 0.93 1.08 0.890
30–40% 1,984 4,871 1.04 0.96 1.12 0.361
40–50% 1,964 4,871 1.03 0.96 1.11 0.425
50–60% 1,950 4,871 1.02 0.95 1.10 0.559
60–70% 1,972 4,871 1.05 0.98 1.14 0.165
70–80% 1,960 4,871 1.05 0.97 1.13 0.203
80–90% 2,019 4,871 1.10 1.02 1.19 0.010
90–100% 2,102 4,871 1.20 1.11 1.29 1.9E-06

Genome-wide polygenic risk scores (gPRS) for T2D were generated in the MVP participants of European ancestry with T2D by calculating a linear combination of weights derived from the Europeans in the DIAMANTE Consortium using the prune and threshold method in PRSice-2 software (pruning r2 = 0.8, P = 0.05). The gPRSs were divided into deciles and the risk of T2D-related vascular outcomes was assessed using a logistic regression model using the lowest decile (0–10%) as the reference category, together with the potential confounding factors of age, gender, and the first 10 PCs of European ancestry. The decile-specific P-values are shown in the column labeled P. In a separate logistic regression analysis, the continuous PRS was set as the dependent variable together with age, gender, and the first 10 PCs, and the P-value for linear trend is shown in the column labeled P for linear trend. For coronary heart disease, a CHD PRS (from CardiogramplusC4DplusUKBB) is included in the regression model as an additional covariate. For acute ischemic stroke, a stroke PRS (from MEGASTROKE consortium) is included in the regression model as an additional covariate. T2D, type 2 diabetes; PRS, polygenic risk score; n Cases, number of cases with the respective vascular outcome; n Controls, number of unaffected controls for the respective vascular outcome; OR, odds ratio; CI, confidence interval.