FIGURE 8.

The effect of media composition on glucose metabolism and insulin signaling in Huh7 cells. Once confluent, cells were treated with media containing 2% human serum and 11 mM glucose for 7 days before treatment media was added. Treatments consisted of either low fat low sugar (LFLS; 11 mM glucose + 200 μM fatty acids), low fat high sugar (LFHS; 200 μM fatty acids + 11 mM glucose + 5.5 mM fructose) or high fat high sugar (HFHS; 800 μM fatty acids + 11 mM glucose + 5.5 mM fructose); all treatments contained 0.5 nM insulin. (a) Media lactate was measured as a marker of anaerobic glycolysis, results are corrected to protein content (lactate); n = 6. (b) Glucose remaining in the media after 24 hr in culture was measured. (c) Phosphorylation of Akt (ser473) was measured by ELISA and confirmed with Western blotting; n = 3. Data are mean ± SEM, *p < .05, **p < .001; two‐way ANOVA with Bonferroni's multiple comparisons test