. 2020 Jul 8;8(13):e14482. doi: 10.14814/phy2.14482

TABLE 1.

The effect of media composition on gene expression in Huh7 cells

	LFLS	LFHS	HFHS
FA and TAG synthesis genes
ACACA	1.08 ± 0.08^a	1.02 ± 0.06^a	0.72 ± 0.02^b
ACACB	0.96 ± 0.04	0.89 ± 0.06	0.93 ± 0.04
DGAT1	0.91 ± 0.06	0.92 ± 0.07	0.79 ± 0.02
DGAT2	0.98 ± 0.06	1.09 ± 0.05	0.97 ± 0.05
FASN	0.88 ± 0.12	1.12 ± 0.17	0.87 ± 0.10
SCD	1.07 ± 0.06	1.31 ± 0.12	1.00 ± 0.10
FA elongation and desaturation genes
ELOVL2	1.05 ± 0.04	0.98 ± 0.06	0.91 ± 0.04
ELOVL5	1.01 ± 0.01^a	0.96 ± 0.03	0.89 ± 0.03^b
ELOVL6	1.02 ± 0.09	1.05 ± 0.09	0.94 ± 0.05
FADS1	0.86 ± 0.03^a	1.07 ± 0.06^b	0.85 ± 0.05^a
FADS2	0.97 ± 0.04	1.14 ± 0.06^a	0.87 ± 0.04^b
Lipid droplet protein genes
BSCL2	0.79 ± 0.02	0.82 ± 0.04	0.80 ± 0.04
CIDEB	0.91 ± 0.11	1.06 ± 0.11	0.96 ± 0.07
CIDEC	0.91 ± 0.09	0.92 ± 0.11	0.99 ± 0.11
PLIN1	0.79 ± 0.05	0.98 ± 0.10	1.04 ± 0.09
PLIN2	0.95 ± 0.05	1.02 ± 0.05	0.99 ± 0.04
PLIN3	0.95 ± 0.04	1.06 ± 0.08	0.98 ± 0.07
PLIN5	0.78 ± 0.05	0.89 ± 0.05	0.92 ± 0.12
Hepatic nuclear receptors & transcription factors
MLXIPL	0.95 ± 0.03	1.09 ± 0.08	0.97 ± 0.07
NR1H3	0.94 ± 0.03	1.07 ± 0.07	0.90 ± 0.06
NR1H4	0.93 ± 0.05	0.96 ± 0.06	0.91 ± 0.05
SREBF1	0.96 ± 0.06	1.15 ± 0.05^a	0.81 ± 0.04^b

Data are mean ± SEM, n = 6. Different letters denote significant differences between means, p < .05; two‐way ANOVA with Bonferroni's multiple comparisons test.