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. 2020 Jun 12;39(28):5124–5137. doi: 10.1038/s41388-020-1346-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Quantitation of phospho-(Ser/Thr) ATM/ATR substrate levels in lysates from MCF10A cells transfected with the indicated siRNA and incubated in the presence or absence of 2.5 mM HU for the indicated times. The intensities of the four substrate bands of interest were quantified using ImageJ. Bars indicate the mean intensity ± SD. b Immunoblotting of MCF10A cell lysates following treatment with 2.5 mM HU for the indicated time points. c, d EdU incorporation in MCF10A cells 72 h post-transfection with the indicated siRNA and treatment with 2.5 mM HU for 12 h. EdU was added for the last 30 min. c Histograms of EdU intensity distribution. d Percentage of EdU+ cells. Data are presented as the mean ± SD (n = 2).