FIGURE 6.

PsGH7a is required for full virulence of P. sojae. (A) P6497 wild-type and transformants hyphae plugs infected wounded hypocotyls, the pictures were taken at 3 days later. (B) P6497 and transformants hyphae plugs infected soybean leaves, the pictures were taken at 3 days later. (C) Determination of P. sojae DNA content in infected hypocotyls. Hypocotyls were collected at 3 days after infection with P6497 and transformants, and DNA was extracted for real-time PCR. (D) Determination of P. sojae DNA content in leaves. Leaves were collected 3 days after infection with P6497 and transformants, and DNA was extracted for real-time PCR. Three independent replicates of each real-time PCR.