TABLE 1.

Expression cassettes and efficiency of RNAi in S. cerevisiae.

siRNA precursor	Cassettes for RNAi		Silencing efficiency	References
	Pathway	siRNA precursor
hairpin RNA	pRS404-TEF1p-Ago1 and pRS405-TEF1p-Dcr1	pRS403-GAL1p-GFP (275 bp)-RAD9 intron 1-GFPrc	strong silencing	Drinnenberg et al., 2009
	p415-TDH3p-Dicer-TEF1p-Ago2	pRS414-TDH3p-YFP (200 bp)-intron-YFPrc	93% and 80% for highly and weakly expressed genome-integrated YFP	Crook et al., 2014
	pRS404-PTEF-Ago1(x2)-pRS405-PTEF-Dcr1(x2)	PFUS1J2- ARO7sense-rad9linker- ARO7antisense-pRS413; PFUS1J2-ZWF1/CDC19sense-rad9linker-ZWF1/CDC19antisense- pRS406	97% for ARO7, 87.8% for CDC19, insignificant for ZWF1	Williams et al., 2015a
	pX-3-AGO1-DCR1 TDH3p:ZWF1↓:can1; pX-3-AGO1-DCR1 RNR2p:ZWF1↓:can1	pX-3-AGO1-DCR1 TDH3p:ZWF1↓:can1; pX-3-AGO1-DCR1 RNR2p:ZWF1↓:can1	95% or 80% for TDH3p or RNR2p-drived shRNA expression, respectively	Kildegaard et al., 2019
dsRNA	pRS404-TEF1p-Ago1 and pRS405-TEF1p-Dcr1	pRS403-GAL1p-GFP (275 bp)-URA3prc	intermediate silencing	Drinnenberg et al., 2009
	pRS-delta-KanMX-LoxP-TEF1p-AGO1-PGK1t-TPI1p-DCR1-GPD1t	pRS416-GPDtrc-TEF1p-GFP (1–180 bp region)-TPI1prc- PGK1t;	∼80%	Si et al., 2014
	p415-TDH3p-Dicer-TEF1p-Ago2	pRS414-TDH3p-RAD9 intron-YFP (200 bp)-RAD9 intron rc-TEF1prc	84% for strongly expressed YFP, 94% for weakly expressed YFP	Crook et al., 2016
antisense RNA	pAG413Gal-Ago2, pAG416Gal-Dicer, pAG415Gal-TRBP	pAG424Gal-AS-GFP	80%	Suk et al., 2011
	pRS-delta-KanMX-LoxP-TEF1p-AGO1 -PGK1t-TPI1p-DCR1-GPD1t	pRS406-TEF1p-AS GFP-PGK1t	∼95%	Si et al., 2014