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Perfusion cell culture set-up using an inclined settler. Cells are recirculated in a loop using a peristaltic pump at a flow rate of 35 mL/min. At the top of the inclined settler, another peristaltic pump harvests cell-free medium. The addition of fresh medium through the feeding pump allows maintaining the working volume at steady state. Blue and red arrows indicate the flow direction (water recirculation) in the heat exchanger.
