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. 2020 Jul 2;8:672. doi: 10.3389/fbioe.2020.00672

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Copyright © 2020 Coronel, Gränicher, Sandig, Noll, Genzel and Reichl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth and metabolism of AGE1.CR.pIX cells in perfusion mode using a stirred-tank bioreactor coupled to an inclined settler or an ATF system. Cultivations with an inclined settler (IS): IS1 (), IS2 (), IS3 (), IS4 (), IS5 (), and IS6 (). Cultivation with the ATF system (). (A) Viable cell concentration (filled symbols) and cell viability (empty symbols). (B) Doubling time (t_d) during the cell growth phase. (C) Cell-specific glucose consumption rate (q_glc) during perfusion (after 48 h). (D) Lactate yield based on glucose consumption (Y_lac/_glc) during perfusion (after 48 h).

Inline graphic — Growth and metabolism of AGE1.CR.pIX cells in perfusion mode using a stirred-tank bioreactor coupled to an inclined settler or an ATF system. Cultivations with an inclined settler (IS): IS1 (), IS2 (), IS3 (), IS4 (), IS5 (), and IS6 (). Cultivation with the ATF system (). (A) Viable cell concentration (filled symbols) and cell viability (empty symbols). (B) Doubling time (t_d) during the cell growth phase. (C) Cell-specific glucose consumption rate (q_glc) during perfusion (after 48 h). (D) Lactate yield based on glucose consumption (Y_lac/_glc) during perfusion (after 48 h).