TABLE 1.

Strains, plasmids and primers.

Strain, plasmid, gene or primer	Relevant characteristics	Source or reference
E. coli DH5α	fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17	New England Biolabs Inc.
E. coli BL21(DE3) pLysS	fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5	New England Biolabs Inc.
pET16bP	pET16b with additional multiple cloning site; allows production of recombinant proteins with N-terminal Histidine10-Tag and gene expression induction with IPTG	Tischler et al., 2009
pET16bP-TagalU	pET16bP vector with recombinant UDP-glucose-pyrophosphorylase gene of T. agreste DSM 44070 (TagalU)	Eurofins Genomics
pET16bP-fw	5′-CATCACAGCAGCG GCCATATCGAAG-3′	This study
pET16bP-rev	5′-CAGCTTCTTTTC GGGCTTTGTTAG-3′	This study