TABLE 2.
Initial tested conditions for the TaGalU reaction and comparison of observed enzyme activities.
| Temperature | TaGalU concentration | Observed TaGalU activity |
| 10°C | 0.013 μg ml–1 | 213 U mg–1 |
| 0.04 μg ml–1 | 42.4 U mg–1 | |
| 0.08 μg ml–1 | 15.6 U mg–1 | |
| 8 μg ml–1 | 11.2 U mg–1 | |
| 22°C | 8 μg ml–1 | 61.7 U mg–1 |
| 30°C | 8 μg ml–1 | 68.4 U mg–1 |
| 50°C | 0.013 μg ml–1 | 1861 U mg–1 |
| 0.04 μg ml–1 | 395 U mg–1 | |
| 0.08 μg ml–1 | 157 U mg–1 | |
| 80°C | 0.013 μg ml–1 | n.d. |
| 0.04 μg ml–1 | n.d. | |
| 0.08 μg ml–1 | n.d. |
Reaction solutions contained 2 mM UTP, 2 mM G1P, 4 mM MgCl2, 50 mM Hepes, pH 7.0, indicated amounts of TaGalU and reaction was carried out in 1 ml at mentioned temperatures. Best conditions and highest specific activity is written in bold. n.d., not detectable.