Table 2.

Diverse mutation events occurred at the target site of AtPDS3 gene (gRNA1) in 15 independent mutants generated using the CRISPR/Cas9 vector version IV

Wild-type (WT) sequence of target site; deletions are indicated by red dashed lines, insertions or substitutions are indicated by red font with plus ‘ + ’ and “s”s sign, respectively; PAM (CGG) is indicated by green underlined italicized font. Mutation events were analyzed using TA-cloning and Sanger sequencing