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. 2020 Feb 21;22(4):1021–1030. doi: 10.1007/s11307-020-01481-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4. — Flow cytometric analysis of a tumors and b spleens harvested from control, XRT-, and XRT + anti-CTLA-4-treated mice on day 8 following treatment initiation (N = 6/group for tumor, N = 5–6/group for spleen). c Representative immunohistochemical staining of CD8a⁺ in paraffin-embedded tumors and spleens harvested from control, XRT-, and XRT + anti-CTLA-4-treated mice on day 8 following treatment initiation. Data are presented as mean ± SEM. The significance level is indicated by asterisks (*). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. XRT, external radiation therapy.