Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 14;154(1):55–75. doi: 10.1007/s00418-020-01861-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — Proliferation of icIg⁺ cells. a–d Visualisation of IgD⁺ cells (polyclonal Abs, brown) followed by detection of Ki-67 (mAb MIB-5, blue). a, b There are several Ki-67⁺icIgD⁺ plasmablasts in the dark zone and Ki67⁻icIgD⁺⁺ PCs in the basal light zone. The icIg⁺ cells in the dark zone do not exhibit the typical nuclear morphology of PCs. c, d In another follicle of the same individual, even more weakly IgD⁺ cells with Ki-67 expression are seen in the dark zone. It is impossible to distinguish intracellular and surface staining. Such GCs are infrequent. e Several dark zone Ki-67⁺ cells stained blue in the first step show icIgM in the second step (brown) of staining (vertical arrows). Single double-positive cells always occur at the surface of the dark zone (horizontal arrow). f In the outer T-cell region of a human spleen Ki-67 (blue) is demonstrated in about 50% of icIgM⁺ migratory plasmablasts (brown). The unstained structure in the centre of the image is a bending central artery. Same method as in e. The crypt epithelium is located beyond the left margin in a, c and below the bottom of the image in e. a–e 38-year-old female. f 17-year-old male. Scale bars 50 µm (a, c), 10 µm (b, d), and 40 µm (e, f)