Table 2.
Double-staining experiments
| First Ag (Ab) | Second Ag (Ab) |
|---|---|
| CD3 (polyclonal) | CD38 (SPC32) |
| CD3 (polyclonal) | CD27 (137B4) |
| CD3 (polyclonal) | CD30 (Ber-H2) |
| CD20 (L26)a | CD3 (polyclonal) |
| CD20 (L26) | CD271 (EP1039Y) |
| CD20 (L26) | CD38 (SPC32) |
| CD20 (L26)a | AID (ZA001) |
| CD20 (L26)a | CD30 (Ber-H2) |
| CD38 (SPC32) | IgM (polyclonal) |
| CD38 (SPC32) | IgD (polyclonal) |
| CD38 (SPC32) | IgG (RWP49) |
| CD38 (SPC32) | IgA (polyclonal) |
| CD38 (SPC32) | CD271 (EP1039Y) |
| CD38 (SPC32) | CD27 (137B4) |
| CD38 (SPC32)a | CD23 (1B12) |
| Ki-67 (MIB-5) | IgM (polyclonal) |
| Ki-67 (MIB-5) | IgD (polyclonal) |
| Ki-67 (MIB-5) | IgG (RWP49) |
| Ki-67 (MIB-5) | IgA (polyclonal) |
| Ki-67 (MIB-5) | CD38 (SPC32) |
| Ki-67 (MIB-5) | CD138 (BB-4) |
| Ki-67 (MIB-5) | CD30 (Ber-H2) |
| Ki-67 (MIB-5)a | AID (ZA001) |
| Ki-67 (MIB-5) + CD3 | CD27 (137B4) |
| IgM (polyclonal)a | Ki-67 (MIB-5) |
| IgD (polyclonal)a | Ki-67 (MIB-5) |
| IgG (RWP49)a | Ki-67 (MIB-5) |
| IgA (polyclonal)a | Ki-67 (MIB-5) |
| IgG (RWP49) | IgM (polyclonal) |
| CD23 (1B12) | CD38 (SPC32) |
| CD138 (BB-4) | CD38 (SPC32) |
| IRF4 (EP190) | CD3 (polyclonal) |
| IRF4 (EP190) | CD38 (SPC32) |
| IRF4 (EP190) | CD30 (Ber-H2) |
| CD30 (Ber-H2)a | AID (ZA001) |
| CD271 (EP1039Y) | CD20 (L26) |
| CD38 (SPC32)b | CD20 (L26) |
| AID (ZA001)a | CD3 (polyclonal) |
| AID (ZA001)a | CD38 (SPC32) |
| CD68 (KiM1-P)a | AID (ZA001) |
| CD68 (KiM1-P)a | CD38 (SPC32) |
All staining combinations not marked by superscript letters were detected with BrightVision/HighDef Blue AP or ABC/HighDef Blue Peroxidase in the first step and ABC/DAB in the second step. Ber-H2 was detected by ABC/DAB with and without tyramide amplification
aFirst primary antibody detected by ABC/DAB and second primary antibody by BrightVision/HighDef Blue AP or ABC/HighDef Blue Perox
bFirst primary antibody detected by ABC/DAB and second primary antibody by ABC/HistoGreen