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. 2020 Jul 2;11(7):509. doi: 10.1038/s41419-020-2699-2

Fig. 5. miR-4652-3p directly targets HIPK2.

Fig. 5

a Targetscan, miRWalk, and mirDIP were used to predict target genes of miR-4652-3p. b HIPK2 expression was examined in miR-4652-3p-overexpressing or miR-4652-3p-inhibited cells by western blot, β-actin was used as a loading control. c Luciferase reporter assay was used to determine miR-4652-3p directly targets the 3′UTR of HIPK2. *P < 0.05. d Changes of E-cadherin, Vimentin and HIPK2 expression were detected by western blot in HONE1 and 5–8F cell lines after transfection of miR-4652-3p inhibitor or HIPK2 siRNAs. e Western blot of HIPK2 protein expression in HONE1 and 5–8F cells treated with SPEN siRNAs or corresponding control. β-actin served as a loading control.