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. 2020 Jul 2;11(7):509. doi: 10.1038/s41419-020-2699-2

Fig. 6. SPEN induces miR-4652-3p expression by modulating PI3K/AKT/c-JUN signaling.

Fig. 6

a Predicted c-JUN binding site to the promoter region of miR-4652-3p. b qRT-PCR of c-JUN mRNA expression in HONE1 and 5-8F cell treated with c-JUN plasmids. ***P < 0.001. c Western blot of c-JUN Protein expression in HONE1 cells transfected with c-JUN plasmids. d Transfection of c-JUN plasmids stimulated miR-4652-3p expression in HONE1 and 5–8F cells. ***P < 0.001. e ChIP assay to verify the binding of c-Jun to miR-4652-3p. *P < 0.05. f mRNA expression of miR-4652-3pin HONE1 and 5-8F cell transfected with LY294002. *P < 0.05. g Western blot detection of PI3K, p-PI3K, AKT, p-AKT, c-JUN, and HIPK2 expression in HONE1 and 5-8F cell lines treated with PI3K inhibitor LY294002. β-Actin was used as a loading control. h Graphic abstract depicting a proposed model for a major mechanism of SPEN in promoting NPC cell migration and invasion.