Table 2.
Reproducibility of mNGS assay.
| Organism | Approximate titer Log(CFU or TCID50)/ml |
Log10(IDseq NT reads) | Average | SD | CV% | |||
|---|---|---|---|---|---|---|---|---|
| Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | |||||
| Escherichia coli | 4.0 CFU/ml | 3.42 | 3.83 | 3.52 | 3.83 | 3.65 | 0.21 | 5.87 |
| Haemophilus influenzae | 2.0 CFU/ml | 1.68 | 2.17 | 1.94 | 2.11 | 1.98 | 0.22 | 11.06 |
| Neisseria meningitidis | 6.6 CFU/ml | 5.90 | 6.24 | 6.02 | 6.24 | 6.10 | 0.17 | 2.74 |
| Streptococcus agalactiae | 6.0 CFU/ml | 4.30 | 4.11 | 4.28 | 3.76 | 4.11 | 0.25 | 6.09 |
| Herpes simplex virus 2 | 3.5 TCID50/ml | 5.08 | 5.28 | 5.15 | 5.34 | 5.21 | 0.12 | 2.21 |
SD standard deviation, CV% coefficient of variance %, NT nucleotide, TCID tissue culture infectious dose, CFU colony forming unit.