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. 2020 Jul 8;11(7):514. doi: 10.1038/s41419-020-2718-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c The expression of DAXX was positively correlated with SUMO-1 and SUMO-2/3 using GEPIA. d The STRING database showed that DAXX interact with SUMO-1, 2, 3. e, f Western blot analysis of DAXX and SUMO-2/3 on BGC-823 transfected with DAXX-HA and si-DAXX. The arrow indicated SUMOylation DAXX. g, h DAXX interact with SUMO-2/3 directly using co-IP and IF assay. (Scale bar = 10 μm). i The SUMO-2/3 mRNA expression was significantly decreased after transfected with si-SUMO-2/3 using qRT-PCR. j The DAXX expression was decreased on BGC-823 transfected with si-SUMO-2/3. k The DAXX expression in the cytoplasm and nucleus was decreased on BGC-823 transfected with DAXX-HA and si-SUMO-2/3, especially in the nucleus. *P < 0.05, **P < 0.01, and ***P < 0.001.