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. 2020 Jul 8;10:11213. doi: 10.1038/s41598-020-68178-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vascularity of CAM during incubation. (a) Panels A to P are representative photomicrographs for days 6, 10, 14, and 18 of incubation, showing CAMs that had been exposed to hypoxic and normoxic conditions; (b) the quantitative comparisons of VDI under hypoxic and normoxic conditions, all values are represented as the mean ± standard error. Values labeled with the same letter (a, b) do not differ significantly; values labeled with different letters show significant differences at P < 0.05. Red color represents chickens incubated in hypoxic condition and blue color represent chickens incubated in normoxic condition. CAM chorioallantoic membrane, VDI vascular density index, TC Tibetan chicken, CH Chahua chicken.