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. 2020 Jul 8;10:11197. doi: 10.1038/s41598-020-68144-6

Figure 3.

Figure 3

miR-124 confers greater regulatory activity on Parp-1 3′UTR compared to miR-125b. (A) Schematic representation of Parp-1 mRNA showing the overlapping binding regions of miR-124 and miR-125b in the 3′UTR sequences. Dose-dependent effects of (B) miR-124 and (C) miR-125b on Parp-1 3′UTR-driven luciferase activity. Luciferase activity of lysates prepared from differentiated SH-SY5Y cells co-transfected with pPARP-3′UTR and increased amounts of (B) pmiR-124 and (C) pmiR-125b. Effects of titrating (D) miR-124 against miR-125b and (E) miR-125b against miR-124 on Parp-1 3′UTR activity (D) HEK-293 T cells were co-transfected with Parp-1 3′UTR and pmiR-125b, in the absence of presence of increase amounts of pmiR-124. 24 h post transfection, cellular lysates were prepared and luciferase activity was measured. (E) Luciferase activity of lysates prepared from HEK-293 T cells co-transfected with Parp-1 3′UTR and pmiR-124, in the absence of presence of increase amounts of pmiR-125b. Data presented in (B–E) are mean values of (n = 3) independent experiments conducted in triplicates with error bars representing the ± SEM. *represents p < 0.05, **p < 0.005, ***p < 0.001, and ****p < 0.0005 for statistical significance, whereas ns represents not significant.