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. 2020 Jul 8;11:3412. doi: 10.1038/s41467-020-17179-4

Fig. 3. GGPP regulates signaling through TLR9.

Fig. 3

a Schematic of Ras-dependent signaling cascades downstream of TLR9. b, c IL-10 expression in human B cells stimulated through TLR9 ± inhibition of (b) AKT (AKTi, MK-2206) or (c) ERK (ERKi, SCH772984) (n = 4, pval = 0.005, and 0.001). d Phosphorylation of AKT (ser473) or ERK1/2 (Thr202/Tyr204) over time in human B cells upon stimulation through TLR9± inhibition of geranylgeranyl transferase (GGTi) (representative of n = 3). e IL-10 expression in human B cells stimulated through TLR9± inhibition of GSK3 (GSK3i, CHIR-99021) (n = 9, pval < 0.001). f Phosphorylation of GSK3 (ser9) over time in human B cells upon stimulation through TLR9± inhibition of geranylgeranyl transferase (GGTi-298), relative to unstimulated controls (n = 4, pval = 0.02). g IL-10 and TNF expression in human B cells stimulation through TLR9 ± GGTi ± GSK3i (n = 7, pval = 0.0001). Each data point represents individual donors. All data presented are mean ± SD. Statistical testing in figures in (b), (c), and (e) was done by a paired t test, f using a twoway ANOVA with Sidak’s multiple comparisons test, and g by Friedman’s test with Dunns’s multiple comparisons test **P < 0.01, ***P < 0.001, ****P < 0.0001 and all significant values are shown.