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. 2020 Jul 8;10:11239. doi: 10.1038/s41598-020-67724-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MIRB Nanoparticle Localization to Neural Cells. (a) Fluorescent images of clusters of neurons before exposure to MIRB nanoparticles (0 min, top insert) and after 2 min of MIRB exposure followed by the removal of free particles (2 min, bottom insert). Scale bar is 100 µm. (b) Kinetics of MIRB association with neurons measured by fluorescent images of MIRB nanoparticles. Neurons were incubated with MIRB nanoparticles at either 5 or 20 µg/ml for the specified duration before removal of free particles. Each error bar denotes the standard deviation of the mean values of all ROIs at a given time point and dose.