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. 2020 Jul 2;11:881. doi: 10.3389/fpls.2020.00881

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Copyright © 2020 Gray, Liu and Facette.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stomatal complex types, part 2. All images are imaged via confocal microscopy. Images (i,iii) are full or partial z-projections while image (ii) is a 3D-reconstructed side view through the stomatal pore. For sunken stomata in Agave and gingko (E,F) a lower focal plane containing the guard cells (iii) and a higher focal plane showing epidermal and subsidiary cells (iv) are shown. (A) Kalanchoe spp. (common unknown variety from garden center); anisocytic and heliocytic. (B) Begonia spp. (common unknown variety from garden center) – heliocytic (C) Didierea madagascariensis - unusual type (D) Anacampseros rufescens – tetracytic with four lateral subsidiary cells (E) Agave bracena – tetracytic with 2 lateral and 2 polar subsidiary cells. (F) Ginkgo biloba – cyclocytic. Green = Calcofluor White and Magenta = Direct Red, except for (E,F) where Green = Propidium Iodide and Magenta = Calcafluor White. All scale bars are 15 micrometers.