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. 2020 Jul 2;11:997. doi: 10.3389/fpls.2020.00997

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Copyright © 2020 Wachananawat, Kuroha, Takenaka, Kajiura, Naramoto, Yokoyama, Ishizaki, Nishitani and Ishimizu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of MpRRT1-tagRFP protein in liverwort. Thallus cells in 2-week-old gemmalings of transgenic pro35S:MpRRT1-tagRFP liverworts co-overexpressing Citrine-MpUSE1A (A–C), Citrine-MpSYP4 (D–F), or Citrine-MpSYP3 (G–I) were observed with a laser scanning confocal microscope. The localization of the Citrine-proteins (green) (A, D, G), tagRFP florescence (B, E, H), and their merged images (C, F, I) are shown. In D–I, small white squares are shown at an increased magnification at the upper right. Scale bars, 25 μm.