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. 2020 Jul 2;11:997. doi: 10.3389/fpls.2020.00997

Figure 4.

Figure 4

Expression profiles of MpRRT1 in liverwort. (A) Expression profile of MpRRT1 based on RNA-Seq data. Expression levels of MpRRT1 in the thallus, gemma cup, and midrib were estimated based on the relative abundances of transcripts with the unit fragments per kilobase million (FPKM). The values are presented as the mean of three biologically independent samples with SD. (B) Schematic representation of the structure of the Tak-1 MpRRT1 (Mapoly0033s0138.1) and the adjacent gene locus based on Marpolbase and sequencing results in this study. Orange and white boxes represent predicted exons and UTRs, respectively. Black lines represent introns. A bidirectional arrow indicates the promoter region used in reporter analyses. (C–H) Expression pattern of proMpRRT1:tdTOMATO in gemma. Images of light field (C, F), fluorescence images of tdTOMATO (D, G), and their merged images (E, H) are shown. Magnified images of the white squares in the upper panels are presented in the images in lower panels. White arrowheads indicate the apical notch. (I–L) GUS staining of proMpRRT1:GUS in 11-day-old thalli (I, J), 19-day-old thalli (K), and antheridiophores (L). (J) is a magnified image of the area of the white squares of (I). White arrowhead indicates GUS staining at the apical notch. Scale bars, 100 μm (C–H), 500 μm (J), 2 mm (I, K), and 5 mm (L).