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. 2020 Jul 2;11:997. doi: 10.3389/fpls.2020.00997

Figure 5.

Figure 5

Chemotype and phenotype of the MpRRT1 genome-edited lines. (A) Monosaccharide compositions of thalli in the MpRRT1 genome-edited lines. Trifluoroacetic acid-hydrolysate non-cellulosic fractions from 21-day-old gemmalings of Tak-1 (gray), Mprrt1-1 (red), and Mprrt1-2 (blue) strains were subjected to ion-chromatography. The monosaccharide contents are shown as the percentages of the total amounts of monosaccharides. Values represent means of three biologically independent samples with SD. Asterisks indicate that the value was significantly different from that of Tak-1 by Student’s t-test: *P < 0.05. Ara, arabinose; Rha, rhamnose; Fuc, fucose; Xyl, xylose; Man, mannose; Gal, galactose; Glc, glucose; GalUA, galacturonic acid; GlcUA, glucuronic acid. Nine-day-old gemmalings of Tak-1 (B) and the Mprrt1-1 mutant (C). Gemmalings were grown on medium covered with cellophane to clearly observe rhizoids. Twenty-one-day-old gemmalings of Tak-1 (D) and the Mprrt1-1 mutant (E). Antheridiophores of Tak-1 (F) and the Mprrt1-1 mutant (G). Bars, 2 mm (B, C, F, G) and 1 cm (D, E).