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. 2020 Jul 2;11:997. doi: 10.3389/fpls.2020.00997

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Copyright © 2020 Wachananawat, Kuroha, Takenaka, Kajiura, Naramoto, Yokoyama, Ishizaki, Nishitani and Ishimizu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MpRRT3 and FRB1/AtRRT8 have RG-I rhamnosyltransferase activity. (A, B) SDS-PAGE of the recombinant (A) MpRRT3 and (B) FRB1/AtRRT8 proteins with a C-terminal FLAG tag expressed in tobacco leaves. The proteins were detected by immunoblotting using an anti-FLAG antibody. (C, D) Rhamnosyltransferase activity of (C) MpRRT3 and (D) FRB1/AtRRT8. The recombinant proteins were reacted with 50 µM GR₈-PA and 0.5 mM UDP-Rha at 30°C for 1 h. The upper and lower chromatograms represent after enzyme reactions with the fraction from control proteins and the recombinant proteins.