Table 4.
Amino acids replacements in structural and regulatory proteins of the type III secretion system in P. savastanoi pathovars of woody hosts.
| Protein | Pathovar/Phylogenetic group/strain a | No. of replacements | Position in the protein | Amino acid in Psv NCPPB 3335 b | Amino acid replacement | Type of replacement c |
|---|---|---|---|---|---|---|
| HrpL | Psr | 1 | 63 | His (H) | Gln (Q) | R |
| Psf | 2 | 38 | Gln (Q) | Arg (R) | C | |
| 106 | Gly (G) | Glu (E) | R | |||
| HrpQ | All P. savastanoi (except for Psv-I) | 1 | 195 | His (H) | Arg (R) | R |
| HrcR | Psn | 1 | 24 | Leu (L) | Met (M) | C |
| HrpV | Psv-II and Psn | 1 | 73 | Phe (F) | Val (V) | R |
| HrcC | Psv-II and Psn CFBP 5067 | 1 | 273 | Gly (G) | Val (V) | R |
| Psf | 1 | 579 | Val (V) | Leu (L) | C | |
| Psn (except for Psn CFBP 5067) | 2 | 273 | Gly (G) | Val (V) | R | |
| 699 | Lys (K) | Gln (Q) | C | |||
| HrpD | Psr | 1 | 145 | Ala (A) | Val (V) | R |
| Psn ICMP 16944 | 1 | 123 | Pro (P) | Thr (T) | R | |
| HrcJ | Psf | 1 | 80 | Glu (E) | His (H) | C |
| HrpZ | Psv-II | 1 | 4 | Leu (L) | Phe (F) | R |
| HrpR | Psf | 1 | 2 | Ser (S) | Asn (N) | C |
a
Psv, Psn, Psf, and Psr, P. savastanoi pathovars savastanoi, nerii, fraxini, and retacarpa, respectively. Phylogenetic groups from Figure 2 : Psv-I (Psv NCPPB 3335 and Psv IMCP 1411), Psv-II (Psv ICMP 4352, Psv PseNe107, Psv DAPP-PG722, Psv ICMP 13519, Psv 0485_9, and Psv ICMP 13786), Psn (Psn ICMP 13781, Psn Psn23 and Psn CFBP 5067).
b
Psv NCPPB 3335 was used as reference genome.
c
C and R, replacements to an amino acid with similar or different biochemical properties than the original, respectively.