Table 5.
Novel putative type III effectors identified in Pseudomonas savastanoi.
| T3SS signal in the N-terminus d | Orhology | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession number a | Protein ID | Annotation | Pfam domain b | Size (aa) | hrp value c | A | B | C | Psv | Psn | Psf | Psr |
| PSA3335_RS11510 | WP_032074452 | DUF72 domain-containing protein | PF01904 | 289 | 2577 | + | + | + | + | + | + | + |
| B7R56_RS15315 | WP_057446723 | Hypothetical protein | Not found | 304 | 2558 | + | − | + | − | − | − | + |
PSA3335_RS11510 and B7R56_RS15315 correspond to the Psv NCPPB 3335 and Psr CECT 4861 genomes, respectively.
The Pfam database (https://pfam.xfam.org/).
hrp value, counter variable used to identify hrp boxes in the promoter regions, 500 nucleotides before the start of the gene.
Prediction of N-terminal secretion signal in Hop proteins. A, high percentage of serine in the 50 N-terminal amino acids; B, isoleucine, leucine, valine, alanine, or proline in the third or fourth N-terminal amino acids; C, absence of acidic residues within the 12 N-terminal amino acids (Lindeberg et al., 2005).
Asterisks indicate oleander strains included in pathovar Psv (see Figure 4 ).
Psv, Psn, Psf and Psr, P. savastanoi pathovars savastanoi, nerii, fraxini and retacarpa, respectively; + = presence, - = absence of a protein ortholog in the genomes of all strains included in the corresponding pathovar analyzed in this study Table 1 .