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. 2020 Jul 2;11:638. doi: 10.3389/fphys.2020.00638

FIGURE 6.

FIGURE 6

The effect of SPAK gene deletion on BK channel activity in the principal cells (PCs) of cortical collecting ducts (CCD) isolated from WT and SPAK KO mice. WT and SPAK KO mice were sacrificed, and renal tubules were manually dissected. BK channel activity was recorded from cell-attached single-cell patches on PCs of CCD. (A,B) The representative recording traces in PCs of CCD from WT and SPAK KO mice groups at a holding potential (Vp) of 40 mV, respectively. (C) Summary of data analysis. As shown in (C), in WT mice (n = 8), the fraction of BK channel # over total patches is 0.78 (5 out of 64), whereas in SPAK KO mice (n = 5), the fraction of BK channel # over total patches is less than 0.001 (0 out 73), p < 0.05. These data suggested that BK channel activity is markedly reduced and absent in SPAK KO mice compared to WT mice. Note: N: channel number; Po: channel open probability; and NPo: channel activity.