Table 1.
Potential antimicrobial peptides or proteins identified in the draft genome sequence of Paenibacillus polymyxa Kp10 using BAGEL4a, antiSMASHb, and CD-Searchc.
| No | Predicted antimicrobial peptides (Protein ID/Locus tag) | Location (contig number) | Amino acid sequence | Theoretical isoelectric point (pI) | Theoretical molecular weight (Da) |
|---|---|---|---|---|---|
| 1 | Lanthipeptide paenibacillina,b (WP_080561132.1/ DBL67_20770) | 12 | MKVDQMFDLDLRKSYEASELSPQASIIKTTIKVSKAVCKTLTCICTGSCSNCK | 9.27 | 3105.79 |
| 2 | Lanthipeptide paenilana,b (WP_019686785.1/DBL67_RS08735) | 3 | MKNQFDLDLQVAKNEVAPKEVQPASGLICTPSCATGTLNCQVSLSFCKTC | 7.57 | 2638.10 |
| 3 | Lasso peptide paeninodinb (WP_013369856.1/ DBL67_10215) | 3 | MSKKEWQEPTIEVLDINQTMAGKGWKQIDWVSDHDADLYNPS | 4.56 | 2502.68 |
| 4 | Sacti5/Sactipeptidea (WP_080561119.1/DBL67_15195) | 5 | MRKLVKRSTNVGDTIEAFGCGCSCYCPCSCYCAGSLTRSSNTSRESDGSYRRDNGTGIGNY | 7.73 | 4455.81 |
| 5 | Sacti10/Sactipeptidea (Not being annotated) | 10 | MDVLVKSAVQVSAHCPFHGSSGCTLSCSYKQ | 7.97 | 1942.17 |
| 6 | Thiazole/oxazole-modified microcins b (PTU47115.1/DBL67_10885) | 3 | MATEVLQTQVIQKAWEDASFREKLMADPKSAIRDVLGVVIPDHIQIKTVEETSDQFYLVIPPNPSGVLATSQKPRSMW | 5.18 | 8781.12 |
| 7 | Histone-like DNA binding protein HUc (PTU44600.1/DBL67_22290) | 15 | MNKTDLINNISSKSGLSKRDVEAVLNGVLGEITDALASGDKVQLIGFGTFETRKRSSRTGRNPQTGNTIEIPESTVPAFKAGNKLKEAVN | 9.52 | 9603.83 |
| 8 | Histone-like DNA binding protein HUc (PTU48889.1/DBL67_03415) | 1 | MLNKTDLINQVSESTELSKKDVTKAIDAVFEAIAGALQNGDKVQLVGFGNFEVRERSARKGRNPQTGEEIEIPASKIPAFKPGKALKDGIK | 9.00 | 9854.26 |
Theoretical isoelectric point and molecular weight of mature peptides (1–5; bold) were computed using Compute pI/MW tool of ExPAsy (Bioinformatics Resource Portal). Cleavage site of paenibacillin, paenilan, and lasso peptide were determined based on previous research (He et al., 2008; Zhu et al., 2016; Park et al., 2017). Cleavage site of sactipeptides and thiazole/oxazole modified microcins were predicted using SignalP 5.0. There was no cleavage site predicted in thiazole/oxazole modified microcins and histone-like DNA binding protein HU.