FIG 3.

Phylogenetic analysis based on 16S rRNA gene sequences of verrucomicrobial representatives. The 16S rRNA gene sequence of the here-assembled “Ca. Udaeobacter” sp. MAG is highlighted with a red diamond and clusters together with the ribosomal clone DA101, affiliated with “Ca. Udaeobacter.” Besides the 16S rRNA gene sequence of the MAG assembled in this study, the 16S rRNA gene sequences of 22 other verrucomicrobial representatives were considered. The tree was rooted on the 16S rRNA gene sequence of Escherichia coli K-12 MG 1655 (NC_000913.3:4035531-4037072). Red, blue, and purple branches indicate the verrucomicrobial classes Verrucomicrobiae, Methylacidiphilae, and Opitutae, respectively. Accession numbers are given in parentheses. Bootstrap values based on 500 replicates are shown at the branching points, and the bar represents 0.05 changes per nucleotide position. All positions with less than 90% site coverage were eliminated. Ca. Xiphinematobac., “Candidatus Xiphinematobacter”; Roseib., Roseibacillus.