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. 2020 Jul 7;11(4):e00972-20. doi: 10.1128/mBio.00972-20

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Copyright © 2020 de Vries et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Epitope mapping for CD4^Xreact1 and CD8^Xreact1. Overlapping peptide-pulsed autologous B-LCL were used to determine the minimal epitope recognized by CD4^Xreact1 and CD8^Xreact1 in IFN-γ ELISPOT. (A and D) Overlapping 15-mer peptides with 11 overlap were initially used. (B and E) Fine-tuning of recognized region using overlapping 10-mer peptides with 9 overlap. (C and F) Confirmation of recognition of F^129–137 (F^AQITAGIAL) by both TCC. MeV, measles; F, fusion protein; SFC, spot-forming cells.