FIG 4.

Isolation of a novel F^129–137-specific TCC. (A) AQITAGIAL^PE tetramer fluorescence-activated cell sorting (FACS) staining of TCC CD8^Xreact1 confirming that the TCC is CD8⁺ and recognizes F^AQITAGIAL in the context of HLA-B*15:01. (B) CD3^APCCy7 and AQITAGIAL^PE tetramer staining of PBMC obtained from three human HLA-B*15:01-positive donors. CD3⁺ CD8⁺ T cells that were positively stained by the HLA-B*15:01-AQITAGIAL tetramer were single cell sorted by FACS and clonally expanded from donor 3. (C) AQITAGIAL^PE tetramer FACS staining of TCC CD8^Xreact2 confirming that the TCC is CD8⁺ and recognizes F^AQITAGIAL in the context of HLA-B*15:01.