TABLE 1.
Lipase 1 is proviral for a range of mammalian and avian viruses in primary human and avian fibroblastsa
| Virus | Host | Subtype | Avg fold titer increase (±SD) |
|||
|---|---|---|---|---|---|---|
| CEF cells |
NHLF cells |
|||||
| MOI 0.01 | MOI 3 | MOI 0.01 | MOI 3 | |||
| A/Puerto Rico/8/34 | Human | H1N1 | 14.2 (3.2) | 11.4 (4.1) | 5.72 (2.7) | 4.69 (2.9) |
| A/Equine/Miami/63 | Horse | H3N8 | 12.6 (3.3) | 9.85 (5.1) | 7.50 (4.7) | 2.32 (0.9) |
| A/Udorn/307/72 | Human | H3N2 | 14.7 (5.3) | 10.4 (0.78) | 5.73 (2.2) | 3.60 (1.9) |
| A/Duck/England/62 | Duck | H4N6 | 207.2 (88.8) | 13.8 (6.1) | 6.94 (3.2) | 11.25 (5.4) |
| A/Turkey/Canada/63 | Turkey | H3N2 | 57.7 (32.6) | 64.6 (38.5) | Not tested | Not tested |
| A/Mallard/Netherlands/10/99 | Duck | H1N1 | 54.4 (29.1) | 15.5 (6.1) | Not tested | Not tested |
a
CEF or NHLF cells were infected with the indicated viruses at a MOI of 0.01 or 3, and 300 nM rlipase 1 was added immediately after inoculum removal. Samples were harvested at 24 h (MOI of 3) or 48 h (MOI of 0.01) and infectious viral titers determined by plaque assay. Data are expressed as the average (± standard deviation) fold increase in titer compared to parallel samples incubated without trypsin and represent results from 3 independent experiments.