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Nissle and EHEC coinfection. (A) Experimental timeline. HIOs were injected with 103 CFU of Nissle. After 12 h, half were challenged with 103 CFU of EHEC. (B) Plot of the mean number of CFU ± SD (n = 3) determined at the indicated times. (C) Cryosection of coinfected HIOs at 42 h (30 h after challenge with EHEC) stained for DNA (blue; DAPI), bacteria (green; anti-O157), and F-actin (red; phalloidin). Bar, 20 μm. A representative image of an experiment performed in triplicate is shown.
