FIG 5.

Nissle and UPEC coinfection. (A) Experimental timeline. HIOs were injected with 10³ CFU of Nissle, and 24 h later, half were challenged with 10³ CFU of UPEC. (B) Plot of the mean number of CFU ± SD (n = 3) determined at the indicated times. (C) Cryosection of coinfected HIOs at 96 h (72 h after challenge with UPEC) stained for DNA (blue; DAPI), bacteria (green; anti-E. coli), and F-actin (red; phalloidin). Bar, 20 μm. A representative image of an experiment performed in triplicate is shown.